TACGenomics is a genomic service company based in California USA, providing comprehensive solution to the problem of handling data generated by NGS devices.
- 1-(424)-361-5063
- service@tacgenomics.com
- 425 Broadway, Santa Monica, CA 90401 USA
scRNA-seq (single cell RNA sequencing) examines the sequence information from individual cells with optimized next-generation sequencing technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. scRNA-Seq provides deeper insight to the multi-tiered complexity of different cells within the same tissue type. TACGenomics use the cutting-edge pipeline to analyze your scRNA-seq data. We put more attention to quality control and data visualization.
Convert the fastq to the gene expression count matrix using 10X cellranger
Quality Control and low quality cell removal
Data normalization and variance modelling for each sample
Data integration using Harmony/MNN method
Clusters generation and marker gene identification of each cluster
Cell type identification by marker genes(manual) and/or singleR method (automatic)
Data visualization by Umap/Tsne/Dotplot/Violin/Heatmap
Gene expression comparison of the same cell type between samples
Trajectory analysis (if applied)
cell-cell communication analysis (if applied)
Single-Cell regulatory Network Inference and Clustering (if applied)
TACGenomics is a genomic service company based in California USA, providing comprehensive solution to the problem of handling data generated by NGS devices.